Proximate compositions, bioactive compounds and antioxidant activity from large-leafed mangrove (Bruguiera gymnorrhiza) fruit

Antioxidants are compounds that can inhibit or prevent the oxidation of the easily oxidized substrate. One of the plants as a potential source of bioactive compounds and antioxidant activity is large-leafed mangrove (Bruguiera gymnorrhiza). This plant was commonly found in the Pacific region of Southeast Asia, Ryukyu Islands, Micronesia and Polynesia (Samoa) to subtropical regions of Australia and has been used by the society. This study aimed to determine the proximate compositions, bioactive compounds and antioxidant activity from large-leafed mangrove fruit which extracted by methanol. It have high carbohydrate content is 29.28%, 66.39% moisture, 2.11% protein, 1.07% fat and 1.15% ash. Old fruit yield greater than young fruit with value 9.94% and 6.83%, respectively. Old fruit has a more effective antioxidant activity (13.47 ppm) compared to young fruit (81.60 ppm) and classified as a very strong antioxidant (IC50 < 50 ppm). Bioactive compounds that act as antioxidants are phenol group. By TLC chromatography technique produces the best eluent, namely methanol and water (4:1) and result 3 fractions. Fraction III had the most effective antioxidant activity with IC50 value of 26.69 ppm.